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Comparison of Peripheral Leukocytes in Japanese Black and Holstein Calves

2002; Canadian Veterinary Medical Association; Volume: 55; Issue: 12 Linguagem: Inglês

10.12935/jvma1951.55.789

2186-0211

Hiromichi OHTSUKA, Shoh-ichi KOMATSU, Satoru Konnai, Shigeo Fukuda, Yoshio Kiku, Tomoo YOSHINO, Masateru Koiwa, Seiichi Kawamura,

Microbial infections and disease research

To evaluate cellular immunity in Japanese Black (JB) calves, changes in peripheral leucocytes were com-pared in two groups of calves–JB calves (JB group;n=10) and Holstein calves (Hol group; n=10). In both groups flow cytometric analysis (FCM) was used within 3 days (0 week) and 3, 6, 9, and 12 weeks after birth.Forward (FSC) and side-scatter (SSC) dot-plots were examined and used to construct monocyte-lymphocytes gates of 3 sizes (small, medium, and large). In both groups, numbers of medium (M)-ML were low in 0 week but increased after birth. In the JB group, however, numbers of M-ML decreased at from 6 to 9 weeks and were significantly lower than those in the Hol group from 6 weeks after birth. Numbers of small (S)-ML in the JB group peaked at 3 weeks and were significantly higher than in the Hol group at 0, 3, 9, and 12 weeks. FCM analysis showed that numbers of CD3+ cells in the JB group were significantly higher than those in the Hol group at 3 weeks after birth. These results indicate that cellular immunity in JB calves differs from that in Hol calves because of changes depending on the role of the T cell.