Rapid and Semiautomated Method for the Analysis of Veterinary Drug Residues in Honey Based on Turbulent-Flow Liquid Chromatography Coupled to Ultrahigh-Performance Liquid Chromatography–Orbitrap Mass Spectrometry (TFC-UHPLC-Orbitrap-MS)
2013; American Chemical Society; Volume: 61; Issue: 4 Linguagem: Inglês
10.1021/jf3048498
ISSN1520-5118
AutoresM.M. Aguilera-Luiz, Roberto Romero‐González, Patricia Plaza‐Bolaños, José Luis Martı́nez Vidal, Antonia Garrido Frenich,
Tópico(s)Bee Products Chemical Analysis
ResumoA simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography–Orbitrap mass spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na2EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 μg/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were <25% for most compounds. Limits of quantification (LOQs) ranged from 5 to 50 μg/kg and limits of identification (LOIs) from 0.1 to 50 μg/kg. The developed method was applied in honey samples, and it was fast and nonlaborious.
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