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Association between single-nucleotide polymorphisms of fatty acid synthase gene and meat quality traits in Datong Yak (Bos grunniens)

2015; Research Foundation of Ribeirão Preto; Volume: 14; Issue: 1 Linguagem: Inglês

10.4238/2015.march.30.21

1676-5680

Mingxing Chu, Xiaoyun Wu, Xian Guo, Jie Pei, Fengping Jiao, Hao-Lin Fang, Chunian Liang, Xuezhi Ding, Pengjia Bao, Peisheng Yan,

Peroxisome Proliferator-Activated Receptors

Fatty acid synthase (FASN) is a key enzyme in fatty acid anabolism that plays an important role in the fat deposit of eukaryotic cells.Therefore, in this study, we detected 2 novel single-nucleotide polymorphisms (SNPs) in the FASN gene in 313 adult individuals of Datong yak using polymerase chain reaction-single strand conformation polymorphism and DNA sequencing techniques.SNP g.5477C>T is located in intron 3 of FASN, and 3 genotypes, HH, HG, and GG, were detected in this mutation site.SNP g.16930T>A is located in exon 37 of FASN, and 2 genotypes, EE and EF, were detected in this site.Association analysis of these 2 SNPs with meat quality traits showed that in SNP g.5477C>T, yaks with the HH genotype and HG genotype ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 14 (1): 2617-2625 (2015) had significantly higher intramuscular fat content than individuals with the GG genotype (P < 0.01).In SNP g.16930T>A, yaks with the EE genotype also had significantly higher IMF content than individuals with the EF genotype (P < 0.01).The results indicate that FASN may be used as a candidate gene affecting intramuscular fat content in Datong yaks.