Combined microcomputed tomography, biomechanical and histomorphometric analysis of the peri-implant bone: a pilot study in minipig model
2016; Elsevier BV; Volume: 32; Issue: 6 Linguagem: Inglês
10.1016/j.dental.2016.03.025
ISSN1879-0097
AutoresMatteo Gramanzini, Sara Gargiulo, Fernando Zarone, Rosario Megna, Antonio Apicella, Raffaella Aversa, Marco Salvatore, Marcello Mancini, Roberto Sorrentino, Arturo Brunetti,
Tópico(s)Bone Tissue Engineering Materials
ResumoTo present a practical approach that combines biomechanical tests, microcomputed tomography (μCT) and histomorphometry, providing quantitative results on bone structure and mechanical properties in a minipig model, in order to investigate the specific response to an innovative dental biomaterial.Titanium implants with innovative three-dimensional scaffolds were inserted in the tibias of 4 minipigs. Primary stability and osseointegration were investigated by means of insertion torque (IT) values, resonance frequency analysis (RFA), bone-to-implant contact (BIC), bone mineral density (BMD) and stereological measures of trabecular bone.A significant positive correlation was found between IT and RFA (r=0.980, p=0.0001). BMD at the implant sites was 18% less than the reference values (p=0.0156). Peri-implant Tb.Th was 50% higher, while Tb.N was 50% lower than the reference zone (p<0.003) and they were negatively correlated (r=-0.897, p=0.006).μCT increases evaluation throughput and offers the possibility for qualitative three-dimensional recording of the bone-implant system as well as for non-destructive evaluation of bone architecture and mineral density, in combination with conventional analysis methods. The proposed multimodal approach allows to improve accuracy and reproducibility for peri-implant bone measurements and could support future investigations.
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