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PACAP ameliorates oxidative stress in the chicken inner ear: An in vitro study

2010; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fnins.2010.10.00269

1662-4548

Bóglárka Rácz,

Cardiovascular, Neuropeptides, and Oxidative Stress Research

Event Abstract Back to Event PACAP ameliorates oxidative stress in the chicken inner ear: An in vitro study Andrea Tamás1*, Gabriella Horváth1, Dóra Reglodi1, Balázs Gasz2, Péter Kiss1, Ferenc Gallyas Jr3, Balázs Sümegi3, Gábor Tóth4, Adrienne Németh5, Kata Habon5, Réka Brubel1, Andrea Lubics1 and Boglárka Rácz3 1 University of Pecs, Department of Anatomy, Hungary 2 County Hospital Zalaegerszeg, Cardiac Surgery, Hungary 3 University of Pecs, Department of Biochemistry and Medical Chemistry, Hungary 4 University of Szeged, Department of Medical Chemistry, Hungary 5 University of Pecs, Department of Oto-rhino-laryngology, Hungary Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide. Numerous studies prove that PACAP has neuroprotective effects in diverse neuronal systems in vitro and in vivo. The involvement of PACAP in visual and olfactory sensory processing has also been documented, but little is known about its effects in the auditory system. The presence of PACAP and its receptor, the specific PAC1 receptor, has been shown in the cochlea and in brain structures involved in auditory pathways. The aim of the present study was to investigate whether PACAP is protective in cochlear oxidative stress-induced cell death, which is known to play a role in several ototoxic insults. Chicken cochlear cells were exposed to 1 mM H2O2, which resulted in a marked reduction of cell viability and a parallel increase of apoptotic and necrotic cells assessed by MTT test, annexin V/propidium iodide flow cytometry and JC-1 apoptosis assay. Co-incubation with 100 nM PACAP increased cell viability and reduced the percentage of apoptotic cells. Furthermore, oxidative stress increased the activation of caspase-3, while simultaneous PACAP treatment reduced it. In summary, our present results demonstrate that PACAP effectively protects cochlear cells against oxidative stress-induced apoptotic cell death. This work was supported by the Hungarian Science Research Fund (OTKA F67830, K72592, CNK78480), ETT, Pécs University Medical School 2009 Grant, Bolyai Scholarship and Gedeon Richter Foundation. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Sensory and motor systems Citation: Tamás A, Horváth G, Reglodi D, Gasz B, Kiss P, Gallyas Jr F, Sümegi B, Tóth G, Németh A, Habon K, Brubel R, Lubics A and Rácz B (2010). PACAP ameliorates oxidative stress in the chicken inner ear: An in vitro study. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00269 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 May 2010; Published Online: 06 May 2010. * Correspondence: Andrea Tamás, University of Pecs, Department of Anatomy, Pecs, Hungary, andreatamassz@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Andrea Tamás Gabriella Horváth Dóra Reglodi Balázs Gasz Péter Kiss Ferenc Gallyas Jr Balázs Sümegi Gábor Tóth Adrienne Németh Kata Habon Réka Brubel Andrea Lubics Boglárka Rácz Google Andrea Tamás Gabriella Horváth Dóra Reglodi Balázs Gasz Péter Kiss Ferenc Gallyas Jr Balázs Sümegi Gábor Tóth Adrienne Németh Kata Habon Réka Brubel Andrea Lubics Boglárka Rácz Google Scholar Andrea Tamás Gabriella Horváth Dóra Reglodi Balázs Gasz Péter Kiss Ferenc Gallyas Jr Balázs Sümegi Gábor Tóth Adrienne Németh Kata Habon Réka Brubel Andrea Lubics Boglárka Rácz PubMed Andrea Tamás Gabriella Horváth Dóra Reglodi Balázs Gasz Péter Kiss Ferenc Gallyas Jr Balázs Sümegi Gábor Tóth Adrienne Németh Kata Habon Réka Brubel Andrea Lubics Boglárka Rácz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.