In vitro Activities of Thiadiazine Derivatives against Leishmania amazonensis

2011; Thieme Medical Publishers (Germany); Volume: 55; Issue: 04 Linguagem: Inglês

10.1055/s-0031-1296850

ISSN

1616-7066

Autores

Lianet Monzote, Ana Margarita Montalvo, Lisset Fonseca, Rolando Pérez, Margarita Suárez, Hortensia Rodrı́guez,

Tópico(s)

Phenothiazines and Benzothiazines Synthesis and Activities

Resumo

Ten thiadiazine derivatives were tested in vitro for antiparasitic effects against both extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis. The results showed that the evaluated compounds exhibited a strong antiproliferative activity on all developmental stages of the parasite. The minimal inhibitory concentration and the 50 % effective concentration values against the promastigote were 2.1-5.1 microg/ml and 0.6-1.8 microg/ml, respectively. The tested compounds caused an irreversible inhibition of the promastigote growth either after 1 h of treatment with 10 microg/ml or after 24 h with 1 microg/ml. Also, the thiadiazine derivatives were active against amastigotes producing between 12 and 89 % of reduction of infection at 100 ng/ml. However, the compounds exhibited high toxicity and provoked inhibition of the phagocytosis in the murine host cell.

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