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Expressions and Roles of AMIGO Gene Family in Vascular Endothelial Cells

2012; Linguagem: Inglês

10.7763/ijbbb.2012.v2.58

2010-3638

Sharmin Hossain, Minhaz Uddin Ahmed, Shamiul Alam, Akuo Watanabe, Ai Harashima, Hideto Yonekura, Hiroshi Yamamoto,

Nitric Oxide and Endothelin Effects

In this study, we have developed shRNA expression vector and determined their RNAi activity. The endothelial cells (ECs) were transfected with the Amphoterin-induced gene and ORF (AMIGO-2) expression vector or AMIGO-2 shRNA expression vector using hemagglutinating virus of Japan (HVJ) Envelope Vector and then the cells were assayed for AMIGO-2 mRNA levels and its survival. In our study, here we have performed expression of AMIGO gene family in primary cultured human vascular cells, and found predominant expression on human microvascular endothelial cells (HMVEC). The expression of AMIGO-2 gene in HMVEC under hypoxia, showed AMIGO-2 gene decreased significantly, suggested that AMIGO-2 maybe involved in vascular remodeling. Based on our study of AMIGO-2 down regulation and over expression showed the down regulation appeared to cause cell death of ECs, and over expression appeared to protect EC death due to reactive oxygen species. Finally, our this study suggest that AMIGO-2 may have an important role in the vascular system as a cell survival promoting factor for vascular ECs, probably as being involved in vascular development, angiogenesis and/or vascular remodeling. (where x can be any amino acid and L could also be replaced by V, I or F). The LRRs are protein-protein interaction motifs and are found in a large number of proteins (2). Some LRR-containing plasma membrane proteins are expressed almost exclusively in the brain, implying specific functions in the central nervous system. Expression analyses indicate that AMIGO-1 is specifically detected in axonal fibers and tracts in the brain. AMIGO-2 and AMIGO-3 expressions are more widespread but are also brain-enriched. Members of the AMIGO family exhibit both homophilic and heterophilic binding, which suggest that they function as novel cell adhesion molecules in neurons. Immobilized ectodomain of AMIGO-1 promoted neurite extension of cultured hippocampal neurons, but, when added to the medium, the same soluble AMIGO-1 inhibited fasciculation of neurites.