Portal de Periódicos da CAPES

The occurrence of intranuclear inclusion bodies in certain tissues of the rabbit inoculated directly with the virus of herpes labialis

1923; SAGE Publishing; Volume: 20; Issue: 7 Linguagem: Inglês

10.3181/00379727-20-195

1535-3702

Ernest W. Goodpasture, Oscar Teague,

Bartonella species infections research

B. Lipschütz has described in experimental herpetic keratitis of rabbits intranuclear, acidophilic bodies, staining readily with eosin, variable in size and shape but usually round or oval and separated from the nuclear membrane by a narrow clear space. They may appear homogeneous or faintly granular. He found these bodies constantly in the nuclei of the epithelial cells and considered them pathognomonic of this lesion. Rarely similar bodies were encountered in the nuclei of connective tissue cells of the lesion. He succeeded in demonstrating these bodies also in the conjunctiva of the inoculated rabbit's eye and in herpetic vesicles of human skin. We have attempted to determine in what tissues of the rabbit the virus of herpes labialis will take as determined by the presence of the above intranuclear bodies at or near the sit of inoculation. The procedure adopted was as follows: A virus obtained from a herpetic vesicle of the human lip was inoculated upon the scarified cornea of a rabbit and was transferred from cornea to cornea at two- or three-day intervals. The intranuclear bodies were found characteristically in corneas thus inoculated. Purulent secretion was collected twenty-four hours after inoculation of a cornea, suspended in saline solution and injected in small quantities with a hypodermic syringe into rabbits as follows: directly into the testicle, into the brain after trephining the skull, and into the abdominal organs after laparotomy. The tracheal mucosa, the abdominal skin and the muco-cutaneous border of the lip were scarified and purulent conjunctival secretion was rubbed into the scarifications. The inoculated areas were excised twenty-four hours after inoculation, fixed in Zenker's solution and stained with haematoxylineosin and with methylene-blue-eosin. Characteristic intranuclear bodies like those described by Lipschütz have been found in the brain, trachea, testicle, adrenal, liver, muco-cutaneous border of the lip and skin of the abdomen.