Portal de Periódicos da CAPES

The Effects of Flk-2/flt3 Ligand as Compared with c-kit Ligand on Short-Term and Long-Term Proliferation of CD34+ Hematopoietic Progenitors Elicited from Human Fetal Liver, Umbilical Cord Blood, Bone Marrow, and Mobilized Peripheral Blood

1996; Mary Ann Liebert, Inc.; Volume: 5; Issue: 6 Linguagem: Inglês

10.1089/scd.1.1996.5.655

2168-6556

F.L. Shapiro, Bronislaw Pytowski, Shahin Rafii, Luc de Witte, Dan Hicklin, Tzy‐Jyun Yao, M A Moore,

Chronic Myeloid Leukemia Treatments

The Flk-2/flt3 ligand (FL) was evaluated and compared with c-kit ligand (KL) for its in vitro proliferative effects on CD34+ cells from human fetal liver, umbilical cord blood, bone marrow, and mobilized peripheral blood. Using a 7-day liquid culture system, FL in combination with interleukin-3 (IL-3), interleukin-6 (IL-6), and granulocyte colony-stimulating factor (G-CSF) was comparable with KL in combination with IL-3, IL-6, and G-CSF for the expansion of hematopoietic progenitors. When FL-containing cultures were assayed after 21 or 28 days, a greater number of progenitors were generated as compared with KL-containing cultures. Using bone marrow microvascular endothelial cells as support stroma, cultures supplemented with FL generated a greater number of progenitors in both the nonadherent and adherent layers at day 35. These data suggest that FL ligand, in combination with other cytokines, can be used for short-term ex vivo expansion of hematopoietic progenitors and facilitates the preservation and possible expansion of primitive cells capable of long-term generation of progenitors.