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Separation, Purification, and Characterization of Analogues Components of a Commercial Sample of New Fuchsin

2010; Oxford University Press; Volume: 48; Issue: 8 Linguagem: Inglês

10.1093/chromsci/48.8.618

1945-239X

María N. Montes de, I. M. Aiassa, María Noel Urrutia, Gustavo A. Argüello, Cristina S. Ortiz,

Lipid Membrane Structure and Behavior

New Fuchsin (NF), also known as Magenta III, has potential applications in photodynamic therapy.The commercial product labeled NF contains two other dye components in different proportions, Magenta II and Magenta I (Rosaniline).The proportions of NF, Magenta II, and Magenta I determined by reversed-phase high-performance liquid chromatography (RP-HPLC) in the commercial sample used were 71.6 ± 0.4%, 25.2 ± 0.2%, and 2.8 ± 0.1% (n = 7), respectively.The isolation, purification, and characterization of commercial NF dye components were carried out applying different techniques, such as preparative column liquid chromatography (PCLC), thin layer chromatography (TLC), RP-HPLC, absorption spectrophotometry, nuclear magnetic resonance spectroscopy (NMR), electrospray ionization mass spectrometry (ESI-MS), and tandem electrospray ionization mass spectrometry (ESI-MS-MS).After separation and isolation, the degree of purity obtained for NF compound was higher than 95% and 92% for Magenta II and Magenta I compounds, respectively.Therefore, it is essential to ensure a high degree of purity of these dyes as raw material to obtain new drugs intended for therapeutic treatments.Cationic dyes are being investigated because there is evidence suggesting that many tumor cells have a major capability to accumulate lipophilic cationic dyes attributable to the more negative mitochondrial membrane potential in comparison with normal cells (6-9).The current and potential uses of dyes have led to further detailed studies on their chemical properties and their impact on the bioenvironment.Scientific progress has led to the development of numerous synthetic drugs; therefore, it is essential to deal with high purity dyes as raw material in order to obtain new drugs for medical applications.Several authors observed that not all the commercial dye batches have acceptable quality (10).For this reason, the purification, isolation, and characterization of analogues of New Fuchsin (NF) commercial sample was designed (Figure 1) because they were not previously described in the literature.For many routine staining procedures, the composition and purity of NF (Magenta III) is probably of less importance.