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Direct measurement of intracellular pressure

1991; American Physical Society; Volume: 260; Issue: 3 Linguagem: Inglês

10.1152/ajpcell.1991.260.3.c652

1522-1563

Suzanne Kelly, Peter T. Macklem,

3D Printing in Biomedical Research

The feasibility of using the servo-null technique for direct measurement of intracellular pressure (Pin) was investigated. A large cell, the Xenopus laevis oocyte, was chosen for study, and it was established that Pin obtained with this method was both stable and accurate in these cells. Median resting Pin in oocytes was found to be 0.27 cmH2O, range 0.14-0.61 cmH2O. During osmotic swelling Pin increased, in a non-linear fashion, to a value of 4.11 cmH2O, range 2.61-8.91 cmH2O, with increases in cell volume (Vc) of 24 +/- 3% (SE). This technique may be of use in the study of cellular mechanics.