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Submitochondrial localization of rat liver .BETA.-alanine-oxoglutarate aminotransferase.

1987; Center for Academic Publications Japan; Volume: 33; Issue: 6 Linguagem: Inglês

10.3177/jnsv.33.439

1881-7742

Nanaya TAMAKI, S Fujimoto, Chizuru MIZOTA, Mariko KIKUGAWA,

Biopolymer Synthesis and Applications

beta-Alanine-oxoglutarate aminotransferase from rat liver was mainly distributed in the mitochondrial fraction, while beta-ureidopropionase, the last one of uracil-metabolizing enzymes to beta-alanine, was predominantly distributed in the cytosolic fraction. When rat liver mitochondria were separated into submitochondrial fractions, beta-alanine-oxoglutarate aminotransferase was localized in the mitochondrial matrix. beta-Alanine was transported into mitochondria with time and the influx of beta-alanine into the matrix was 0.47 nmol/mg of mitochondrial protein at 2 min after incubation.