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The use of nanosized hydroxyapatite on silk fibroin hydrogels to improve osteointegration

2016; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fbioe.2016.01.02446

2296-4185

Marta Ribeiro, De Moraes Mariana, Beppu Marisa, Fernandes Maria, Monteiro Fernando, Ferraz Maria,

Periodontal Regeneration and Treatments

Event Abstract Back to Event The use of nanosized hydroxyapatite on silk fibroin hydrogels to improve osteointegration Marta Ribeiro1, 2, 3, Mariana A. De Moraes4, 5, Marisa M. Beppu4, Maria H. Fernandes6, Fernando J. Monteiro1, 2, 3 and Maria P. Ferraz7 1 i3S - Instituto de Investigação e Inovação em Saúde, Portugal 2 INEB - Instituto de Engenharia Biomédica, Portugal 3 FEUP - Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Metalúrgica e Materiais, Portugal 4 School of Chemical Engineering, University of Campinas, Brazil 5 UNIFESP - Federal University of São Paulo, Department of Exact and Earth Sciences, Brazil 6 Faculdade de Medicina Dentária, Universidade do Porto, Laboratory for Bone Metabolism and Regeneration, Portugal 7 University Fernando Pessoa, Energy, Environment and Health Research Unit/Biomedical Research Center, Portugal Introduction: The development of scaffolds to repair bone defects is the main purpose in bone tissue engineering. The use of hydrogels as synthetic extracellular matrix is drawing a great attention due to their high water content and consequent viscoelastic and diffusive transport properties[1]. In this context, a hydrogel, that will act as a temporary matrix must be cytocompatible and have a number of biological functions actively encouraging cells ingrowth[2],[3]. Silk fibroin (SF) is a natural polymer which possesses important properties for bone tissue engineering, including biocompatibility, biodegradability, high permeability to oxygen and water vapor, and low-cost[4]. This work aimed to investigate the influence of the addition of nanosized hydroxyapatite (nanoHA) on SF hydrogels, and to evaluate cell-matrix surface interactions. Materials and Methods: The cocoons of Bombyx mori were degummed with Na2CO3 solution to remove the sericin and the SF fibers were dissolved in a ternary solvent of CaCl2:CH3CH2OH:H2O. Composite hydrogels were obtained using an innovative technique where the dry powder of nanoHA aggregates was first mixed with ethanol at 70 vol% and then slowly mixed with the SF aqueous solution, prior to hydrogel formation. The hydrogel structure was investigated by SEM, XRD and FTIR. For the biological characterization, human Bone Marrow Stromal Cells (hBMSCs) were seeded on the top of each hydrogel and cultured up to 21 days. The cells-seeded hydrogels were assessed for cell viability and proliferation, and observed by CLSM. Results and Discussion: Morphological study revealed that the composite hydrogels presented both macro- and microporous structures, providing good conditions for cell proliferation and diffusion of nutrients and metabolites. The nanoHA particles were embedded throughout the SF matrix (Figure 1A). Both XRD and FTIR spectra showed that composite hydrogels simultaneously exhibited characteristic peaks from nanoHA and a predominant structure of silk II. Concerning the biological properties, the increase of metabolic activity and proliferation of hBMSCs on hydrogels over time indicates the cytocompatible and non-toxic nature. The higher metabolic activity and proliferation was achieved when the hBMSCs were cultured on composite hydrogels with nanoHA, indicating that the nanoHA may play a major role in metabolic cell activities and proliferation. These results were well correlated with confocal images that showed extensive cell spreading and proliferation on the hydrogels, and at day 21 cells completely covered the surface of SF/nanoHA hydrogels with a dense cell layer (Figure 1B). Conclusion: In the present work it was shown that the addition of nanoHA on SF hydrogels allowed optimizing biological properties, improving hBMSCs attachment, viability and proliferation. This combination of macro- and microporous material and biological properties provide a promising hydrogel for bone tissue engineering for temporary supporting the formation of new tissue. FEDER funds through the Programa Operacional Factores de Competitividade – COMPETE; Portuguese funds through FCT – Fundação para a Ciência e a Tecnologia in the framework of the NaNOBiofilm project (PTDC/SAUBMA/111233/2009) and PhD grant (SFRH/BD/90400/2012); Project 346/13 CAPES (Brazil)-FCT (Portugal) Call 21/2012, and CNPq (Brazil); Fluidinova S A (Maia, Portugal) for the supply of nanosized hydroxyapatite powder; Bratac (São Paulo, Brazil) for the supply of cocoons of Bombyx mori silkwormReferences:[1] Ahearne M, et al. J R Soc Interface 2005;2:455-63.[2] Tortelli F, et al. Eur Cell Mater 2009;17:1-14[3] Hutmacher DW, et al. J Tissue Eng Regen Med 2007;1:245-60.[4] Ribeiro M, et al. Biomatter 2014; 4:e28536. Keywords: Bone Regeneration, Hydrogel, composite, matrix-cell interaction Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Synthetic scaffolds as extracellular matrices Citation: Ribeiro M, De Moraes MA, Beppu MM, Fernandes MH, Monteiro FJ and Ferraz MP (2016). The use of nanosized hydroxyapatite on silk fibroin hydrogels to improve osteointegration. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02446 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers' terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marta Ribeiro Mariana A De Moraes Marisa M Beppu Maria H Fernandes Fernando J Monteiro Maria P Ferraz Google Marta Ribeiro Mariana A De Moraes Marisa M Beppu Maria H Fernandes Fernando J Monteiro Maria P Ferraz Google Scholar Marta Ribeiro Mariana A De Moraes Marisa M Beppu Maria H Fernandes Fernando J Monteiro Maria P Ferraz PubMed Marta Ribeiro Mariana A De Moraes Marisa M Beppu Maria H Fernandes Fernando J Monteiro Maria P Ferraz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.