STRUCTURE OF THE HEMAGGLUTININ GENE OF H0N1 STRAIN OF HUMAN INFLUENZA VIRUS AND ITS EXPRESSION IN ESCHERICHIA COLI
1981; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-515080-4.50017-1
AutoresAlan R. Davis, Debi P. Nayak, Masahiro Ueda, Alan L. Hiti, Dennis G. Kleid, Donald Dowbenko,
Tópico(s)RNA and protein synthesis mechanisms
ResumoABSTRACT The structure of the hemagglutinin gene of the H0N1 strain of human influenza virus has been compared to those of other strains. It was found that the H0 hemagglutinin showed the highest homology to the H2 hemagglutinin, suggesting a close geneological relationship between the two. To express the H0 viral HA in Escherichia coli it was placed under control of the lac operon of E. coli. To achieve a high level of expression it was necessary to fuse the H0 hemagglutinin to the E. coli protein β-galactosidase. Fused proteins containing selected portions of HA were specifically precipitated by anti WSN antiserum and therefore, contained antigenic determinants.
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