Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Genetic diversity of Yersinia pestis in Brazil

2012; Research Foundation of Ribeirão Preto; Volume: 11; Issue: 3 Linguagem: Inglês

10.4238/2012.september.25.10

1676-5680

Maria Betânia Melo de Oliveira, Maria Paloma Silva de Barros, V.M. Silveira-Filho, M.R. Araújo-Nepomuceno, Valdir de Queiroz Balbino, Nilma Cintra Leal, Alzira Maria Paiva de Almeida, Tereza Cristina Leal‐Balbino,

Bee Products Chemical Analysis

Plague outbreaks are occasionally reported in Brazil.Unfortunately, due to great genetic similarity, molecular subtyping of Yersinia pestis strains is difficult.Analysis of multiple-locus variable number of tandem repeats (VNTR), also known as MLVA, has been found to be a valuable tool to discriminate among strains.To check for genetic differences, strains obtained from two different ecological complexes in Brazil collected during two different epidemiological events, an epizootic in Sítio Alagoinha in 1967 and an outbreak in Planalto da Borborema in 1986, were subtyped through MLVA using 12 VNTR loci.Three clusters (A, B and C) were observed.Of the 20 strains from the epizootic, 18 fit into cluster A. Cluster A was divided into two subgroups: A 1 (15 strains) and A 2 (3 strains).Of the 17 strains from the outbreak, 15 fit into cluster B. Cluster B was divided into three subgroups: B 1 (4 strains), B 2 (4 strains) and B 3 (7 strains).Cluster C is a singleton with one epizootic strain.The external standards, Y. pestis CO92 and Y. pseudotuberculosis IP32953, formed two clusters of singletons.The stability of 12 VNTR loci of three unrelated cultures ©FUNPEC-RP www.funpecrp.com.