Produção Nacional
Revisado por pares
Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi
2016; Springer Nature; Volume: 47; Issue: 2 Linguagem: Inglês
10.1016/j.bjm.2016.01.028
ISSN1678-4405
AutoresLuciane Maria Colla, Andreiza Lazzarotto Primaz, Silvia Benedetti, Raquel Aparecida Loss, Marieli de Lima, Christian Oliveira Reinehr, Telma Elita Bertolin, Jorge Alberto Vieira Costa,
Tópico(s)Biofuel production and bioconversion
ResumoA Plackett-Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p<0.05) on lipase production and were consecutively studied through a Full Factorial Design 2(3), with the concentration of yeast extract and pH being significant (p<0.05). These variables were optimized using a central composite design, obtaining maximum lipolytic activities with the use of 45g/L of yeast extract and pH 7.15. The statistical model showed a 94.12% correlation with the experimental data.
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