Continuous Cell Line Derived from the Kidney of Yamame, Oncorhynchus masou
1978; Springer Science+Business Media; Volume: 44; Issue: 5 Linguagem: Inglês
10.2331/suisan.44.415
ISSN1349-998X
AutoresTasuku Watanabe, Naoki Kobayashi, Yoshihiko Sato, Y Ishizaki,
Tópico(s)Aquaculture Nutrition and Growth
ResumoA continuous cell line was established from the kidney of Yamame, Oncorhynchus masou. It took 8 weeks at 20°C and 9 weeks at 15°C in primary culture, 9-26 days from secondary to tenth culture, and 7-14 days since 10 passages for the surface of tissue culture bottle to be covered a confluent monolayer of cells. The cell line cultivated at 20°C from primary culture, designated YNK, has been subcultured 50 times over the period of 20 months. The cells of the line multiply in manner of fibroblastic monolayer with 65 chromosomes, and were characterized by the number of glanular intracytoplasmic organellas around nucleus. YNK cells have the permissible temperature range of 4-29°C for growth.
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