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Improved Method for Analysis of Glycated Haemoglobin by Ion Exchange Chromatography

1994; SAGE Publishing; Volume: 31; Issue: 4 Linguagem: Inglês

10.1177/000456329403100409

1758-1001

Solveig Eckerbom, Yngve Bergqvist, Jan‐Olof Jeppsson,

Analytical Methods in Pharmaceuticals

The Pharmacia Mono S column for glycated haemoglobin (HbA 1c ) was compared with Mono S HR 5/5 and three other cation exchange columns to optimize analysis of HbA 1c . This column allows a significant decrease in separation time, resulting in a sample throughput of 12 samples per hour. The choice of parameters for integration of peak areas in the chromatograms is very important for reproducible results. Since pure calibrators of HbA 1c are not available, the relative contribution of HbA 1c to the total amount of haemoglobin is calculated. We have compared valley-to-valley integration with baseline integration to assess reproducibility. Valley-to-valley integration significantly enhances the reproducibility of the method and is therefore preferable for routine analyses of HbA 1c .