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BIBTEX RIS

Diversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern Brazil

2012; Research Foundation of Ribeirão Preto; Volume: 11; Issue: 3 Linguagem: Inglês

10.4238/2012.september.3.3

ISSN

1676-5680

Autores

Erika Abreu, M. L. P. Tinoco, E. C. de Andrade, F. J. L. Aragão,

Tópico(s)

Legume Nitrogen Fixing Symbiosis

Resumo

Eleven isolates of cowpea severe mosaic virus (CPSMV), a member of the genus Comovirus, were selected from 50 samples collected of nine cowpea fields in Northeastern Brazil (Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, and Distrito Federal) and partially sequenced.The RNA1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3C-like protease, and the RNA-directed RNA polymerase genes from CPSMV, had high identity among isolates, varying from 98 to 100%.No evidence was found for intermolecular or intramolecular recombination.Phylogenetic analysis confirmed that the Brazilian CPSMV isolates are substantially different from the CPSMV strain USA.Despite the low variability found among Brazilian CPSMV isolates, there were notable differences in the symptomatology of infected cowpea plants, ranging from mild to moderate.Previous reports have demonstrated an association between CPSMV symptom determinants and helicase.However, we found no correlation between the helicase ©FUNPEC-RP www.funpecrp.com.

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