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Effect of ultra-micro powder qiweibaishusan on the intestinal microbiota and enzyme activities in mice

2012; Elsevier BV; Volume: 32; Issue: 21 Linguagem: Inglês

10.5846/stxb201109271422

1000-0933

Zhoujin Tan, 吴海 WU Hai, 刘富林 LIU Fulin, 蔡莹 CAI Ying, 蔡光先 CAI Guangxian, 张华玲 ZHANG Hualing, 曾奥 ZENG Ao,

PDF HTML阅读 XML下载 导出引用 引用提醒 超微七味白术散对肠道微生物及酶活性的影响 DOI: 10.5846/stxb201109271422 作者: 作者单位: 湖南中医药大学 湖南 长沙 中国,湖南中医药大学09级研究生 湖南 长沙中国,湖南中医药大学 湖南 长沙 中国,湖南中医药大学 湖南 长沙 中国,湖南中医药大学 湖南 长沙 中国,湖南中医药大学10级研究生 湖南 长沙中国,湖南中医药大学10级研究生 湖南 长沙中国 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金(81173214);湖南省科技厅重点项目(2010SK2002);湖南省研究生科研创新项目(CX2010B348) Effect of ultra-micro powder qiweibaishusan on the intestinal microbiota and enzyme activities in mice Author: Affiliation: Hunan University of TCM,Changsha,Hunan,Postgraduate Courses,Hunan University of TCM,Changsha,Hunan,Hunan University of TCM,Changsha,Hunan,Hunan University of TCM,Changsha,Hunan,Hunan University of TCM,Changsha,Hunan,Postgraduate Courses,Hunan University of TCM,Changsha,Hunan,Postgraduate Courses,Hunan University of TCM,Changsha,Hunan Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:肠道微生物与人体多项生理功能密切相关,为探讨超微七味白术散疗效与肠道微生物及酶活性的关系,为中药复方超微饮片临床应用提供科学依据,运用抗生素建立菌群失调小鼠模型,分别灌胃给药七味白术散传统汤剂、超微全量汤剂、超微1/2量汤剂、超微1/4量汤剂、超微1/8量汤剂,分析肠道菌群数及酶活性。结果表明,模型组的肠道微生物(细菌、大肠杆菌、酵母菌、霉菌、乳酸菌及双歧杆菌)数量、木聚糖酶活、蛋白酶活及淀粉酶活均低于对照组(P<0.05),纤维素酶活性高于对照组P<0.01)。传统汤剂组的细菌及酵母菌数均高于对照组,但霉菌数明显下降(P<0.05)。酵母菌数随超微药物浓度的升高而增多,细菌及霉菌数在超微1/2量时最多,与传统汤剂相比,超微1/2组的三类菌均显著增加(P 0.05);经超微全量治疗后,大肠杆菌增长十分迅速,远高于其余各组(P<0.01);七味白术散能促进肠道乳酸菌和双歧杆菌的增殖(P<0.05),恢复肠道菌群平衡。肠道乳酸菌和双歧杆菌的变化趋势一致,传统药物组的数量比对照组明显增加(P<0.01),超微药物组超过了传统汤剂组的数量(P<0.05),超微1/2剂量组的数量最大,远高于其它各组(P<0.01)。肠道内纤维素酶、木聚糖酶、蛋白酶及淀粉酶活性与超微饮片浓度有一定相关性,但肠道酶活性未达到对照组水平(P<0.05)。超微七味白术散1/2量治疗肠道菌群失调小鼠的效果与传统汤剂相当。 Abstract:The physiological functions of the human body are influenced by intestinal microbiota. To provide scientific evidence for the clinical effectiveness of ultra-micro decoction pieces of traditional Chinese medicine (specifically the effect of ultra-micro Qiweibaishusan on intestinal microbiota and enzyme activities), mice were rendered dysbacteriotic via administration of antibiotics and then treated with traditional Qiweibaishusan. The effects of the full amount, 1/2 the amount, 1/4 the amount, and 1/8 amount of ultra-micro Qiweibaishusan on the intestinal microbiota and enzyme activities were analysed. The results showed that the quantities of intestinal microbiota including bacteria, Escherichia coli, yeast, mold, lactobacillus and bifidobacterrium, and the level of xylase activity, protease activity and amylase activity in model group mice were lower than that in control group mice (P<0.05). The intestinal cellulase activity in model group mice was higher than that in control group mice (P<0.01). The quantities of intestinal bacteria and yeast in traditional Qiweibaishusan group mice were higher than that in control group mice, while intestinal mold was lower (P<0.05).The quantity of intestinal yeast increased with the treatment of ultra-micro Qiweibaishusan concentration, while the quantity of intestinal bacteria and mold was highest in the group of mice treated with 1/2 amount of ultra-micro Qiweibaishusan. The quantities of the three categories of intestinal microbiota in the group of mice treated with 1/2 amount of ultra-micro Qiweibaishusan were significantly higher than those in the group of mice treated with the traditional Qiweibaishusan (P 0.05). The quantity of Escherichia coli in the group mice treated with the whole amount of ultra-micro Qiweibaishusan increased very quickly and was far higher than in the other groups (P<0.01).The growth of intestinal lactobacillus and bifidobacterrium could be stimulated by Qiweibaishusan (P<0.05). The quantities change of intestinal lactobacillus and bifidobacterium had the same tendency when the model mice were treated with Qiweibaishusan. And the quantities of intestinal lactobacillus and bifidobacterium in the group mice treated with the traditional Qiweibaishusan increased more obviously than which in the control group mice(P<0.01). The quantities of intestinal lactobacillus and bifidobacterium in the groups mice treated with ultra-micro Qiweibaishusan were higher than those in the group of mice treated with the traditional Qiweibaishusan(P<0.05).And the quantities of intestinal lactobacillus and bifidobacterium in the group treated with 1/2 amount of ultra-micro Qiweibaishusan were far more than that in the other ultra-micro Qiweibaishusan group mice (P<0.01).The activity of intestinal cellulase, xylase, protease and amylase showed some correlation to the concentration of Ultra-micro Qiweibaishusan, while the intestinal enzyme activities in the treated mice were lower than that in control group mice(P<0.05). The curative effect of 1/2 amount of ultra-micro Qiweibaishusan on dysbacteriotic mice was comparable to the effects presented in traditional Qiweibaishusan group. 参考文献 相似文献 引证文献