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Occurrence of TTX in a Brackish Water Puffer “Midorifugu”, Tetraodon nigroviridis, Collected from Thailand

1999; Japanese Society for Food Hygiene and Safety; Volume: 40; Issue: 5 Linguagem: Inglês

10.3358/shokueishi.40.5_363

1882-1006

Yahia Mahmud, Kunio Yamamori, Tamao Noguchi,

Environmental Toxicology and Ecotoxicology

Screening tests were carried out on the toxicity of a brackish water puffer, Tetraodon nigroviridis, collected from Thailand through November, 1996 to May, 1998. Among the different tissues, the skin showed the highest toxicity scores, ranging from 49.2-286MU/g, followed by muscle (5.4-11MU/g), while liver and intestine mostly gave scores under 5MU/g. The toxin was partially purified by three kinds of column chromatography consisting of activated charcoal, Bio-Gel P-2 and Bio-Rex 70. Analyses by HPLC, TLC, electrophoresis, 1H-NMR and LC/MS identified the purified toxin as tetrodotoxin.