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Studies on the In Vitro Antioxidant Activity of Laportea Aestuans Leaf Extract

2014; International organization of Scientific Research (IOSR); Volume: 8; Issue: 1 Linguagem: Inglês

10.9790/2402-08123341

2319-2402

Okereke S.C, I Elekwa,

Phytochemistry and Biological Activities

Hydroethanolic leaf extract of Laportea aestuans L. was screened for antioxidant activity using various antioxidant models such as 1,1-diphenyl-2-picryl hydroxyl quenching (DPPH) assay, superoxide scavenging assay (O 2 oo ), reducing power capacity (RC), scavenging capacity towards hydroxyl ion (OH o ) radical, nitric oxide (NO o ) radical inhibition, hydrogen peroxide inhibition activity (H 2 O 2 ), lipid peroxidation (LPO) inhibition activity and 2,2'-azinobis-3-ethylbenzothiozolin-6-sulfonic acid (ABTS o+ ) cation decolourization test.Total phenolic content, total flavonoid content and ascorbic acid content were also determined.The leaf extract of L. aestuans (LAE) yielded 199.3 ± 4.041mg Gallic acid/100g of sample as the total phenolic content, 90.7 ± 6.807mg Quercetin/100g of sample as total flavonoid content and 52.0 ± 5.000mg vitamin/100g of sample as ascorbic acid content.The extract exhibited high scavenging activity against DPPH, O 2 , OH, NO, H 2 O 2 , ABTS and Lipid peroxide (LPO) with IC 50 values of 15.0 ± 1.00, 247.0 ± 4.58, 84.3 ± 3.79, 67.3 ± 3.06, 230.7 ± 7.02, 81.0 ± 3.61 and 82.7 ± 6.43µg/ml respectively.Correlation was high between polyhenolic content and antiradical potential of different reactive oxygen species indicated that polyphenols are considered as main antioxidants.