Portal de Periódicos da CAPES

NCR+ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures

2015; Nature Portfolio; Volume: 6; Issue: 1 Linguagem: Inglês

10.1038/ncomms9280

2041-1723

Paolo Carrega, Fabrizio Loiacono, Emma Di Carlo, Angelo Scaramuccia, Marco Mora, Romana Conte, Roberto Benelli, Grazia Maria Spaggiari, Claudia Cantoni, Stefania Campana, Irene Bonaccorsi, Barbara Morandi, Mauro Truini, Maria Cristina Mingari, Lorenzo Moretta, Guido Ferlazzo,

Eosinophilic Esophagitis

Tertiary lymphoid structures (TLSs) are a common finding in non-small cell lung cancer (NSCLC) and are predictors of favourable clinical outcome. Here we show that NCR+ innate lymphoid cell (ILC)-3 are present in the lymphoid infiltrate of human NSCLC and are mainly localized at the edge of tumour-associated TLSs. This intra-tumoral lymphocyte subset is endowed with lymphoid tissue-inducing properties and, on activation, produces IL-22, TNF-α, IL-8 and IL-2, and activates endothelial cells. Tumour NCR+ILC3 may interact with both lung tumour cells and tumour-associated fibroblasts, resulting in the release of cytokines primarily on engagement of the NKp44-activating receptor. In patients, NCR+ILC3 are present in significantly higher amounts in stage I/II NSCLC than in more advanced tumour stages and their presence correlate with the density of intratumoral TLSs. Our results indicate that NCR+ILC3 accumulate in human NSCLC tissue and might contribute to the formation of protective tumour-associated TLSs. NCR+type 3 innate lymphoid cells display lymphoid tissue-inducing ability. Here the authors show that these cells are increased in early-stage human lung cancer, respond to cancer cells and associated fibroblasts by producing cytokines and are associated to intratumoural ectopic lymphoid structures.