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SOME CHEMICAL INVESTIGATIONS ON THE STRUCTURE OF INSULIN

1950; Cold Spring Harbor Laboratory Press; Volume: 14; Linguagem: Inglês

10.1101/sqb.1950.014.01.019

1943-4456

Frederick Sanger,

Protein Structure and Dynamics

The problem of protein structure has been studied by two main types of methods. Specialized physicochemical techniques have given much information about the size and shape of molecules, and the newer analytical methods have made possible the accurate determination of the component amino-acids. Very little is known, however, about the order in which these amino-acids are arranged to form the whole protein molecule. This problem can probably best be attacked by the methods of organic chemistry, that is to say, by the gradual and systematic breakdown of these molecules and investigation of the structure of the products of partial degradation. Such an approach was largely hampered in the past by the extreme complexity of any mixture produced by the partial hydrolysis of a protein. The introduction of new chromatographic methods with a very high resolving power has made possible the reinvestigation of this problem, and in this paper an account...