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Improved Synthesis of CMP-Sialates Using Enzymes from Frog Liver and Equine Submandibular Gland

1979; De Gruyter; Volume: 360; Issue: 1 Linguagem: Inglês

10.1515/bchm2.1979.360.1.159

0018-4888

Johan Haverkamp, Jean‐Marie Beau, Roland Schauer,

Peptidase Inhibition and Analysis

An efficient method for the preparation of CMP-N-acetylneuraminic acid using crude or partially purified CTP:N-acylneuraminate cytidylyltransferase from equine submandibular gland or frog liver is described. The yield of the sugar nucleotide after purification by ion-exchange chromatography and gel filtration was 95%. The compound was studied by 360 MHz 1H NMR spectroscopy in addition to the usual chemical and physical analyses. The preparation of radioactive or unlabelled CMP 4-O-methyl-N-acetylneuraminic acid, which is not known to occur in nature, was achieved in 17% yield with the aid of the equine enzyme.