Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae
2016; American Society for Microbiology; Volume: 60; Issue: 6 Linguagem: Inglês
10.1128/aac.02947-15
ISSN1098-6596
AutoresAna P. Silva, Isabel Faria‐Ramos, Elisabete Ricardo, Isabel M. Miranda, Maria José Espinar, Sofia Costa‐de‐Oliveira, Rafael Cantón, Acácio G. Rodrigues, Cidália Pina‐Vaz,
Tópico(s)Vibrio bacteria research studies
ResumoA flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemases.
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