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First Report of Necrotic Streaking of Asiatic Lilies Caused by Plantago asiatica mosaic virus in Chile

2016; American Phytopathological Society; Volume: 100; Issue: 8 Linguagem: Inglês

10.1094/pdis-01-16-0091-pdn

1943-7692

Alexis Vidal, R. Camps, Ximena Besoaín,

Plant Pathogenic Bacteria Studies

HomePlant DiseaseVol. 100, No. 8First Report of Necrotic Streaking of Asiatic Lilies Caused by Plantago asiatica mosaic virus in Chile PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Necrotic Streaking of Asiatic Lilies Caused by Plantago asiatica mosaic virus in ChileA. K. Vidal, R. Camps, and X. BesoainA. K. VidalSearch for more papers by this author, R. CampsSearch for more papers by this author, and X. BesoainSearch for more papers by this authorAffiliationsAuthors and Affiliations A. K. Vidal R. Camps X. Besoain , Escuela de Agronomía, Pontificia Universidad Católica de Valparaíso, Chile. Published Online:26 May 2016https://doi.org/10.1094/PDIS-01-16-0091-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Plantago asiatica mosaic virus (PlAMV) was first detected in Chile by the Servicio Agrícola y Ganadero de Chile (SAG) associated with symptomless Lilium plants in 2013. In Quillota, Chile, a survey was done during spring of 2015 on greenhouse Asiatic hybrid lilies being grown for cut flowers. Symptomatic plants of cvs. Amsterdam, Indian Summerset, Litouwen, and Serengeti were found. Symptoms consisted of brown necrotic streaking associated with irregular leaf chlorosis, and severely infected plants also showed brown streaks in the stem and irregular necrosis in tepal tips. Only in cv. Litouwen it was observed that microbulbs replaced flower buds. From symptomatic plants, RNA was extracted using RNeasy Plant Mini Kit (QIAGEN) and RT-PCR was performed employing primers potex 1 (Gibbs et al. 1998) and potex 3 (van der Vlugt and Berendsen 2002) of gene RdRp. Specific bands for potexvirus were observed (Pájtli et al. 2015). Bands of 400 bp were obtained from all symptomatic plants. PCR products of cvs. Indian Summerset and Amsterdam were sent to Macrogen (Korea) for purification and sequencing. Both sequences obtained revealed 99% nucleotide identity with the consensus sequences of PlAMV from Hungary (LN714022, LN714023). After a positive result for PlAMV, a pathogenicity test was performed on cvs. Indian Summerset and Litouwen, which had previously tested negative for potexvirus. Each PlAMV isolate was prepared in PBS buffer and inoculated onto three replicate 1-month-old plants (15 to 20 cm in height) that had been previously rubbed with carborundum. Three other plants were treated with PBS only as negative controls. Plants were grown in a greenhouse (23 ± 5°C). Forty-five days after inoculation, plants from cv. Indian Summerset showed a shorter stem, necrotic streaks in some leaves associated with chlorosis in irregular patterns, a long brown necrotic streak in the stem, and necrosis in the tepal tips previous to anthesis. Plants of cv. Litouwen showed symptoms of decreased growth, brown necrotic streaks in the leaves associated with chlorosis, and microbulbs instead of flower buds. RNA was extracted from plants as previously described and tested by RT-PCR with a primer for potexvirus (Pájtli et al. 2015) and then confirmed by a second RT-PCR with a primer set specific for PlAMV (Parrella et al. 2015). All symptomatic plants tested positive with both sets of primers and the control plants all tested negative. The two inoculated PlAMV isolates sequences were deposited in GenBank (Accession Nos. KU522243 and KU522244). To our knowledge, this is the first report of Koch’s postulates for PlAMV on Lilium plants worldwide, and the first report of the disease called necrotic streaking of lily caused by PlAMV in Chile affecting Asiatic cultivars. Lilium bulb export is the main ornamental industry in Chile, and this disease could be a risk for this industry.References:Gibbs, A., et al. 1998. J. Virol. Methods 74:67. https://doi.org/10.1016/S0166-0934(98)00070-6 Crossref, ISI, Google ScholarPájtli, E., et al. 2015. Plant Dis. 99:1288. https://doi.org/10.1094/PDIS-01-15-0107-PDN Link, ISI, Google ScholarParrella, B., et al. 2015. Plant Dis. 99:1289. https://doi.org/10.1094/PDIS-03-15-0281-PDN Link, ISI, Google Scholarvan der Vlugt, R. A. A., and Berendsen, M. 2002. Eur. J. Pathol. 108:367. https://doi.org/10.1023/A:1015644409484 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 8 August 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 22 Jul 2016Published: 26 May 2016First Look: 6 Apr 2016Accepted: 28 Mar 2016 Pages: 1799-1799 Information© 2016 The American Phytopathological SocietyCited byPlantago asiatica mosaic virus: An emerging plant virus causing necrosis in lilies and a new model RNA virus for molecular research20 July 2022 | Molecular Plant Pathology, Vol. 23, No. 10Plantago asiatica mosaic virusCABI Compendium, Vol. CABI CompendiumViruses of Some Bulbous Ornamental Flowers from Liliaceae Family14 November 2021Incidence and Occurrence Pattern of Viruses in Lilies (Lilium spp.) on Jeju IslandResearch in Plant Disease, Vol. 25, No. 2Differences in infectivity and pathogenicity of two Plantago asiatica mosaic virus isolates in lilies5 September 2018 | European Journal of Plant Pathology, Vol. 153, No. 3Lilium spp. (Lilium longiflorum, L. lancifolium, L. tsingtauense)6 June 2020Sequence variability between Plantago asiatica mosaic virus isolatesActa Horticulturae, No. 1193Detection of Plantago asiatica mosaic virus in lily hybrid plants (Lilium spp.) in Costa Rica grown from imported bulbs17 November 2017 | Australasian Plant Disease Notes, Vol. 12, No. 1