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Biochemical Studies of Bacterial Derivatives. X. Preparation of Human Tubercle Bacillus Protein MA-100.

1930; SAGE Publishing; Volume: 27; Issue: 7 Linguagem: Inglês

10.3181/00379727-27-4905

1535-3702

Peter Masucci, Kenneth L. McAlpine,

Microbial Metabolic Engineering and Bioproduction

In an effort to produce a potent tuberculin in a concentrated form and with the least possible chemical manipulation, Seibert1 resorted to ultrafiltration, a method based on the conclusion from previous experiments that the active principle in tuberculin which is responsible for eliciting skin reactions in tuberculous guinea pigs is a water-soluble protein of the nature of an albumin. While this appears to be a promising method for concentrating the protein without incurring the possibility of denaturation, the end product is still complex since it retains all of the non-dialyzable constituent of the original filtrate among which are metaproteins, proteoses, and a large proportion of carbohydrate.The aim of this investigation was to determine (1) whether the carbohydrate is linked up with the protein molecule in the form of a glucoprotein, and (2) if not, to prepare a protein fraction free from carbohydrate in order that its biological activity would not be masked by its presence. A method is outlined bel...