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Simultaneous Determination of Plasma Aldosterone, Corticosterone, 11-Deoxycorticosterone, 18-Hydroxydeoxycorticosterone, Cortisol, and 11-Deoxycortisol: Methods and Applications

1976; Oxford University Press; Volume: 66; Issue: 3 Linguagem: Inglês

10.1093/ajcp/66.3.588

1943-7722

Nicholas D. Giordano, Richard J. Koletsky, Simon Koletsky,

Pharmacological Effects and Assays

A method for the simultaneous determination of aldosterone, corticosterone (B), 11-deoxycorticosterone (DOC), 18-hydroxy-11-deoxycorticosterone (18-OH-DOC), cortisol, and 11-deoxycortisol in a single 1-ml sample of plasma is described. The method is applicable to both man and experimental animal. After extraction and purification by thin-layer chromatography (TLC), aldosterone was determined by radioimmunoassay (RIA) employing antibodies to aldosterone: B, DOC, cortisol and 11-deoxycortisol were determined by RIA employing antibodies to corticosterone; 18-OH-DOC was obtained colorimetrically using the Porter-Silber reagent. Recoveries after extraction and chromatography were: aldosterone 71% +/- 9.4 SD; B 81% +/- 10.2; DOC 73% +/- 8.4; cortisol 65% +/- 7.2; 11-deoxycortisol 74% +/- 6.3 using labeled steroids; 18-OH-DOC 82% +/- 8.7 using inert steroid. The method has a high specificity and is reproducible and accurate.