Artigo Acesso aberto Produção Nacional Revisado por pares

Selection of Lipase-Producing Microorganisms through Submerged Fermentation

2010; De Gruyter; Volume: 65; Issue: 7-8 Linguagem: Inglês

10.1515/znc-2010-7-811

ISSN

1865-7125

Autores

Luciane Maria Colla, Andreiza Lazzarotto Primaz, Silvia Benedetti, Raquel Aparecida Loss, Marieli de Lima, Christian Oliveira Reinehr, Telma Elita Bertolin, Jorge Alberto Vieira Costa,

Tópico(s)

Biofuel production and bioconversion

Resumo

Lipases are enzymes used in various industrial sectors such as food, pharmaceutical and chemical synthesis industries. The selection of microorganisms isolated from soil or wastewater is an alternative to the discovery of new species with high enzymes productivity and with different catalytic activities. In this study, the selection of lipolytic fungi was carried out by submerged fermentation. A total of 27 fungi were used, of which 20 were isolated from dairy effluent and 7 from soil contaminated with diesel oil. The largest producers were the fungi Penicillium E-3 with maximum lipolytic activity of 2.81 U, Trichoderma E-19 and Aspergillus O-8 with maximum activities of 2.34 and 2.03 U where U is the amount of enzyme that releases 1 μmol of fatty acid per min per mL of enzyme extract. The fungi had maximum lipolytic activities on the 4th day of fermentation.

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