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Mitochondrial DNA variability in populations of Centris aenea (Hymenoptera, Apidae), a crop-pollinating bee in Brazil

2013; Research Foundation of Ribeirão Preto; Volume: 12; Issue: 1 Linguagem: Inglês

10.4238/2013.march.15.3

ISSN

1676-5680

Autores

Vinina Silva Ferreira, Cândida Maria Lima Aguiar, Eddy José Francisco de Oliveira, Marco Antônio Costa, Gilberto M. M. Santos, Janisete Gomes Silva,

Tópico(s)

Insect and Arachnid Ecology and Behavior

Resumo

Centris spp are oil-collecting solitary bees that are valuable pollinators of crops such as Brazil nut, cashew, and acerola.We investigated the genetic variability of populations of C. aenea in the northeastern region of Brazil.Total DNA was extracted from 59 individuals from 6 locations in the States of Pernambuco and Bahia and a 600-650-bp fragment of the mitochondrial COI/COII region amplified by PCR, followed by digestion with the restriction enzymes DraI and SspI.PCR-RFLP analysis revealed eight different haplotypes among the populations.Haplotype A1, revealed by DraI, was the most frequent (50%), and haplotypes A3 and A4 were exclusive to Feira de Santana, Bahia and Morro do Chapéu, Bahia, respectively.Among the ©FUNPEC-RP www.funpecrp.com.

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