Role of Neutrophil Elastase in Endotoxin-Induced Mucus Hypersecretion in Rat Nasal Epithelium
2000; SAGE Publishing; Volume: 109; Issue: 11 Linguagem: Inglês
10.1177/000348940010901111
ISSN1943-572X
AutoresTakeshi Shimizu, Yukimitsu Takahashi, Yuichi Majima, Kazuhiko Takeuchi, Yasuo Sakakura,
Tópico(s)Salivary Gland Disorders and Functions
ResumoIn the present study, hypertrophic and metaplastic changes of goblet cells were induced in rat nasal epithelium by intranasal instillation of endotoxin or elastase. A significant increase in the amount of intraepithelial mucosubstance was observed after 24 hours during 3 days of instillation. The elastase-induced mucus production was not inhibited in neutrophil-depleted rats, but the endotoxin-induced change was significantly inhibited. Intranasal instillation of the neutrophil elastase inhibitor ONO-5046 partially inhibited the endotoxin-induced mucus production. Epithelial mucus secretion was evaluated by the temporary decrease in the amount of intraepithelial mucosubstance. The endotoxin-induced mucus secretion peaked 3 to 6 hours after intranasal instillation, coinciding with the peak of the intraepithelial neutrophil infiltration. The elastase-induced mucus secretion peaked 1 to 3 hours after intranasal instillation; intraepithelial neutrophil infiltration was not induced by elastase. These results indicate that neutrophil elastase is an important mediator of the intraepithelial mucus synthesis and secretion induced by endotoxin.
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