Studies on the bioactive marine metabolites-IV. Hemagglutinins in the acorn barnacle balanus (Megabalanus) roseus: purification and partial characterization.
1982; Springer Science+Business Media; Volume: 48; Issue: 10 Linguagem: Inglês
10.2331/suisan.48.1421
ISSN1349-998X
Autores Tópico(s)Marine Biology and Environmental Chemistry
ResumoThree different molecular species of agglutinins were found to be persent in the coelomic fluid of Balanus (Megabalanus) roseus. By affinity chromatography on acid-treated Sepharose 4B and subsequent gel filtration on Sephadex G-100, tow of them were obtained in purified forms. A major active component, BRA-3, reacted with rabbit erythrocytes at a concentration as low as 0.36 μg/ml. A minor component, BRA-2 weakly reacted with rabbit blood cells but strongly with human O cells. the molecular weights of BRA-2 and BRA-3 were found to be 120000 and 43000 daltons, respectively. The purified agglutinins were inhibited in varying degrees by galactose lactose, glucuronic acid, galacturonic acid, and N-acetyIneuraminic acid.
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