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Possible oxidative effects of isotretinoin and modulatory effects of vitamins A and C in Saccharomyces cerevisiae

2016; Academic Journals; Volume: 15; Issue: 6 Linguagem: Inglês

10.5897/ajb2015.15050

1684-5315

Silva Lemos Ala iacute de, Leopoldino Coelho Rodrigues Vanessa, Vin iacute cius Oliveira Barros Marcus, Muhammad Torequl Islam, Pablo Sousa de Aguiar Ra iacute, Maria Oliveira Ferreira da Mata Ana, Melo de Carvalho Ricardo, Fernanda Correia Jardim Paz M aacute rcia, Mendes Feitosa Chistiane, eacute lia de Carvalho Melo Ana, Pablo Sousa de Aguiar Ra iacute, eacute lia Carvalho Melo Cavalcante Ana, Pablo Sousa de Aguiar Ra iacute, Maria Oliveira Ferreira da Mata Ana, Fernanda Correia Jardim Paz M aacute rcia, Leopoldino Coelho Rodrigues Vanessa, Muhammad Torequl Islam, Maria Oliveira Ferreira da Mata Ana, Fernanda Correia Jardim Paz M aacute rcia, Mendes Feitosa Chistiane, eacute Carvalho Melo Cavalcante Ana, Muhammad Torequl Islam, Pablo Sousa de Aguiar Ra iacute, Maria Oliveira Ferreira da Mata Ana, Melo de Carvalho Ricardo, Mendes Feitosa Chistiane,

Biocrusts and Microbial Ecology

Isotretinoin (ITN), chemically known as 13-cis-retinoic acid, is a part of the broad group of compounds related to vitamin A. It is particularly employed in the treatment of nodular cystic acne and as an inhibitor of proliferation of neoplastic cells, by exerting a regulatory effect on the cell differentiation. This study aimed at investigating the possible oxidative effects of ITN and modulatory effects of vitamins A and C in mutant and non-mutant Saccharomyces cerevisiae strains. In addition, to reconfirm the oxidative effects, five in vitro antioxidant assays were also prepared taking the alpha-tocopherol analogue, trolox as a standard. In vivo study conducted on S. cerevisiae cells was carried out with ITN 20 µg/ml taking hydrogen peroxide (H2O2) as stressor (STR), whereas ITN 5 to 50 µg/ml was considered for in vitro assays taking similar dose of trolox (TRO). Results show ITN to have oxidative effect in both in vitro and in vivo tests. In conclusion, ITN produced oxidative effects and there may be an hypervitaminosis effect with vitamins A and C, thus insinuation to genetic material. Key words: Assay, isotretinoin, vitamin A, vitamin C, oxidative stress.