Phenotypic Characterization of Natural Killer (NK) Cells in Antiretroviral Naïve Chronically Infected HIV-1 Patients (the CIRCB Afrodec Cohort)
2014; Mary Ann Liebert, Inc.; Volume: 30; Issue: S1 Linguagem: Inglês
10.1089/aid.2014.5253.abstract
ISSN1931-8405
AutoresCarole Stéphanie Sake, Jules Tchadji, Achille Nangue, Nadesh Nji, Georgia Ambada, Edith Temgoua, Samuel Martin Sosso, Rachel Kamgaing, François‐Xavier Etoa, Godwin Nchinda,
Tópico(s)T-cell and B-cell Immunology
ResumoAIDS Research and Human RetrovirusesVol. 30, No. S1 Innate ImmunityFree AccessPhenotypic Characterization of Natural Killer (NK) Cells in Antiretroviral Naïve Chronically Infected HIV-1 Patients (the CIRCB Afrodec Cohort)Carole Stéphanie Sake Ngane, Jules C. Tchadji, Achille Nangue, Nadesh Nji, Georgia Ambada, Edith Temgoua, Samuel Sosso, Rachel Kamgaing, François-Xavier Etoa, and Godwin NchindaCarole Stéphanie Sake Ngane1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonUniversity of Yaoundé I, Department of Biochemistry, Yaoundé, CameroonSearch for more papers by this author, Jules C. Tchadji1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonUniversity of Yaoundé I, Yaoundé, CameroonSearch for more papers by this author, Achille Nangue1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonSearch for more papers by this author, Nadesh Nji1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonSearch for more papers by this author, Georgia Ambada1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonUniversity of Yaoundé I, Yaoundé, CameroonSearch for more papers by this author, Edith Temgoua1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonSearch for more papers by this author, Samuel Sosso1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonSearch for more papers by this author, Rachel Kamgaing1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonSearch for more papers by this author, François-Xavier EtoaUniversity of Yaoundé I, Department of Biochemistry, Yaoundé, CameroonSearch for more papers by this author, and Godwin Nchinda1-CIRCB/Microbiology and Immunology Lab, Yaoundé, CameroonSearch for more papers by this authorPublished Online:30 Oct 2014https://doi.org/10.1089/aid.2014.5253.abstractAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail P12.14Background: Natural Killer (NK) cells are effectors cells of the innate immune system which play a critical role as a first line of defense against viral infection. They are characterized as CD3-CD56+CD16+/− cells and effect cytotoxic activity against target cells as well as cytokines and chemokines production. NK cytotoxicity is modulated by inhibitory and activating receptors. HIV-1 infection maintains immune system in a sustained state of activation and causes immune dysfunction. However the impact of NK cell phenotype modulation has not been acessed in the context of Antiretroviral naïve HIV-1 infection.We shall verify the existence of HIV-specific NK cells in antiretroviral naïve chronically infected HIV-1 patients.Methods: 35 treatment naïve HIV-1 patients, CD4 greater than 350 cell/μl and 30 healthy donors were recruited. Age ranged from 21 to 65 years old. Patients group was composed of 5 patients with Viral load (VL) 4,5 Log. PBMCs were obtained from whole blood using ficoll-paque density grandient. NK cells are analyzed by multiparametric flow cytometry in bulk PBMCs. samples were aquired using BD FACScanto II machine and data analysed by flowjo 7.4Results: There was a differential modulation of NK cell phenotype as we observed a decreased expression of activating markers NKP30, NKP44, NKP46 and a significant increase (P<0,05) in the expression of HLADR, CD38 and NKG2D in treatment naïve HIV-1 infected patients relative to healthy donors. Down regulation of NKG2A expression was also observed in the patients group.Conclusions: This study shows phenotypic pertubations on NK cells in treatment naive HIV-1 infected patients. Observation of increased expression of activating receptors NKG2D, CD38 and HLA-DR is a pathological signature of HIV-1 infection however a decreased NKG2A in expression could be necessary in increase cytolytic function of NK cells.FiguresReferencesRelatedDetails Volume 30Issue S1Oct 2014 InformationCopyright 2014, Mary Ann Liebert, Inc.To cite this article:Carole Stéphanie Sake Ngane, Jules C. Tchadji, Achille Nangue, Nadesh Nji, Georgia Ambada, Edith Temgoua, Samuel Sosso, Rachel Kamgaing, François-Xavier Etoa, and Godwin Nchinda.Phenotypic Characterization of Natural Killer (NK) Cells in Antiretroviral Naïve Chronically Infected HIV-1 Patients (the CIRCB Afrodec Cohort).AIDS Research and Human Retroviruses.Oct 2014.A129-A129.http://doi.org/10.1089/aid.2014.5253.abstractPublished in Volume: 30 Issue S1: October 30, 2014PDF download
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