Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

2016; Elsevier BV; Volume: 154; Linguagem: Inglês

10.1016/j.talanta.2016.03.098

1873-3573

Bruno Alves Rocha, Bruno Ruiz Brandão da Costa, Nayara Cristina Perez de Albuquerque, Anderson Rodrigo Moraes de Oliveira, Juliana Maria Oliveira Souza, Maha Al-Tameemi, Andrés D. Campiglia, Fernando Barbosa,

Analytical chemistry methods development

In this study, a novel method combining dispersive liquid-liquid microextraction (DLLME) and fast liquid chromatography coupled to mass spectrometry (LC-MS/MS) was developed and validated for the extraction and determination of bisphenol A (BPA) and six bisphenol analogues, namely bisphenol S (BPS), bisphenol F (BPF), bisphenol P (BPP), bisphenol Z (BPZ), bisphenol AP (BPAP) and bisphenol AF (BPAF) in human urine samples. Type and volume of extraction and disperser solvents, pH sample, ionic strength, and agitation were evaluated. The matrix-matched calibration curves of all analytes were linear with correlation coefficients higher than 0.99 in the range level of 0.5–20.0 ng mL−1. The relative standard deviation (RSD), precision, at three concentrations (1.0, 8.0 and 15.0 ng mL−1) was lower than 15% with accuracy ranging from 90 to 112%. The biomonitoring capability of the new method was confirmed with the analysis of 50 human urine samples randomly collected from Brazilians. BPA was detected in 92% of the analyzed samples at concentrations ranging <LOQ to 10.4 ng mL−1. The detection rates of BPS (10%), BPAF (4%) and BPF (2%) were much lower than 92%.