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Early biological effect of in vivo gene transfer of platelet-derived growth factor (PDGF)-B into healing patellar ligament

1998; Springer Nature; Volume: 5; Issue: 9 Linguagem: Inglês

10.1038/sj.gt.3300712

1476-5462

Norimasa Nakamura, Konsei Shino, Takashi Natsu-ume, Shuji Horibe, Nobuyoshi Matsumoto, Y Kaneda, T. Ochi,

Periodontal Regeneration and Treatments

To define the early biological effect of in vivo introduction of the PDGF-B gene on the healing of ligaments, a HVJ-liposome suspension containing platelet-derived growth factor (PDGF)-B cDNA was injected directly into the injured patellar ligament of 14-week-old male Wistar rats. Rats were killed at 1, 4 and 8 weeks for the morphological analysis of angiogenesis by laminin immunohistochemistry and of collagen deposition by Masson's Trichrome staining and collagen I immunohistochemistry. PDGF-B gene transfer caused the enhanced expression of PDGF in healing ligament up to 4 weeks after transfection, leading to an initial promotion of angiogenesis and subsequent enhanced collagen deposition in the wound. Enhanced and accelerated matrix synthesis in the PDGF-B gene introduced healing ligament suggests that this gene transfer technique may be a potentially useful tool for improving soft tissue repair.