Regulation of hypoxia-induced mRNA expressions of HIF-1alpha?and osteopontin and in vitro radiosensitization by tirapazamine in human nasopharyngeal carcinoma HNE-1 and CNE-1 cells
2010; BioMed Central; Volume: 29; Issue: 2 Linguagem: Inglês
10.5732/cjc.009.10500
ISSN1944-446X
AutoresPeng Xu, Jianming Huang, Yuan Ren, Xiao Zha, Bi-Fang Deng, Jun-Hui Wu, Jinyi Lang,
Tópico(s)Glioma Diagnosis and Treatment
Resumo窑Nasopharyngeal Carcinoma Column窑Tumor hypoxia is one of the main reasons for radiotherapy and chemotherapy failure in solid tumors (chemotherapy and radiotherapy resistance).The hypoxic cells account for 10%50% in solid tumors and their tolerance to radiation and chemotherapy is 2.53 times stronger than that of aerobic cells, which becomes one of the important factors making cancer difficult to cure, and easy to recur and metastasize.Therefore, the toxic drugs to hypoxic cells in combination with chemoradiotherapy is an important regimen for cancer treatment.Tirapazamine (TPZ) is one of the most remarkable toxic drugs to hypoxic cells.TPZ enters into the cell, forms a high activity of free radicals through the role of intracellular reductase, and reduces the intracellular oxygen ion into oxygen.Under hypoxic condition, the highly active TPZ free radical can obtain a hydrogen atom from the DNA through the mediation of topoisomerase II, cause single/doublestrand breaks and chromosome damage, thus resulting in cell death.Studies have shown that TPZ combined with radiotherapy has synergistic effects on tumor cells, but whether it can downregulate hypoxiainduced genes under hypoxic condition has not been reported 13 .In this experiment, HNE1 EB+ and CNE1 EB human nasopharyngeal carcinoma cells were used as an in vitro model to explore the expression differences of hypoxia inducible factorlα (HIF1α ) and OPN in these two cell lines under normoxic and hypoxic conditions and
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