2, 5-Dimethoxy-2, 5-Dihydro-Furan (DMDF) crosslinked radio-opaque biodegradable antimicrobial sutures
2016; Frontiers Media; Volume: 4; Linguagem: Inglês
10.3389/conf.fbioe.2016.01.00787
ISSN2296-4185
AutoresFrancis Nimmy, Pawar Harpreet, Paulomi Ghosh, Santanu Dhara,
Tópico(s)Orthopedic Surgery and Rehabilitation
ResumoEvent Abstract Back to Event 2, 5-Dimethoxy-2, 5-Dihydro-Furan (DMDF) crosslinked radio-opaque biodegradable antimicrobial sutures Nimmy K. Francis1, Harpreet Pawar1, Paulomi Ghosh1 and Santanu Dhara1 1 Indian Institute of Technology, Kharagpur, School of Medical Science & Technology, India Introduction: Silk offers good tensile strength, excellent handling characteristics and unfailing knot strength as a suture material. Imparting radio-opacity to a biodegradable polymer like silk may opens a wide arena of theranostic applications like post-operative follow-up of arterio-venous fistula, aneurysmal embolization surgeries or orientation markings on biopsy tissues using conventional radiographic techniques[1]. Current methods of radio-opacifying polymers like ligand chelation, use of salts of heavy metals (BaCl3, BaSO4) have undesirable biochemical responses in vivo[2][3]. Materials and Methods: Degummed Bombyx mori silk fibres were treated with differential percentage of crosslinking solution of 2, 5-Dimethoxy-2, 5-Dihydro-Furan (DMDF) –Iodine under high temperature and pressure. Sutures were fabricated, assessed for radio-opacity and straight pull and knot pull strength were tested as per US pharmacopeia guidelines for tensile strength testing of surgical sutures. Antimicrobial testing was also carried out for the sutures. Results and Discussion: Scanning electron microscopy images of treated fibers demonstrated increased surface roughness compared to untreated ones. Spectrophotometric assessment for free NH2 functional group of the silk by Ninhydrin assay determined the optimal DMDF cross-linking concentration[4]. Protolytic cleavage of DMDF to form 2, 3 di iodo butenedial in the presence of Iodine under acidic conditions and interaction of its carbonyl moieties with amine groups of silk (Schiff base reaction) is the proposed mechanism of the reaction[5][6][7]. Radio-opacity assessment of DMDF- Iodine crosslinked silk suture in comparison to untreated ones showed highly visible shadows on X Ray films for crosslinking solution percentage of 300mM Iodine (162.9±17.01). Lead sheet of thickness 0.07mm demonstrated the radio-opacity of 218±4.54 as a control. Cross-linking reduced the straight pull strength from 797.67±28 MPa (100mM I2) to 276.3±29.1 (500mM I2) and knot pull strength from 503.5±0.3Mpa (100mM I2) to 135.8±14.5Mpa (500mM I2). Highest elongation percentage was noted for 100mM Iodine (19.73 %) and least value for 500mM iodine (6.99%). Increase in Iodine concentration resulted in reduction of tensile strength. This may be explained by change in conformation to β-sheet structure and breakage of intermolecular hydrogen bonds at elevated temperature[8][9][10]. Presence of Iodine attributed to the antimicrobial property of crosslinked suture samples. Irregular zone of inhibition was seen around the sample on Staphylococcus aureus petri plates after 24 hrs of incubation. Conclusion: Suggested method of cross-linking delivered significant radio-opacity to the silk sutures without degradation of inherent high mechanical strength along with the additional antimicrobial property. Results of this study may serve as a crucial step for exploration of radio-opaque silk for varied biomedical applications. IIT Kharagpur has been acknowledged for providing infrastructural facility.References:[1] Rajak, S. N.; Habtamu, E.; Weiss, H. A.; Kello, A. B.; Gebre, T.; Genet, A.; Bailey, R. L.; Mabey, D. C.; Khaw, P. T.; Gilbert, C. E.; Emerson, P. M.; Burton, M. J., Absorbable versus silk sutures for surgical treatment of trachomatous trichiasis in Ethiopia: a randomised controlled trial. PLoS medicine 2011, 8 (12), e1001137. DOI: 10.1371/journal.pmed.1001137.[2] Thanoo, B. C.; Jayakrishnan, A., Barium sulphate-loaded p(HEMA) microspheres as artificial emboli: preparation and properties. Biomaterials 1990, 11 (7), 477-81[3] Sabokbar, A.; Fujikawa, Y.; Murray, D. W.; Athanasou, N. A., Radio-opaque agents in bone cement increase bone resorption. The Journal of bone and joint surgery. British volume 1997, 79 (1), 129-34.[4] Friedman, M., Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences. Journal of agricultural and food chemistry 2004, 52 (3), 385-406. DOI: 10.1021/jf030490p[5] Johnson, S.; Dunstan, D.; Franks, G., A novel thermally-activated crosslinking agent for chitosan in aqueous solution: a rheological investigation. Colloid Polym Sci 2004, 282 (6), 602-612. DOI: 10.1007/s00396-003-0985-z[6] Hansen, E. W.; Holm, K. H.; Jahr, D. M.; Olafsen, K.; Stori, A., Reaction of poly(vinyl alcohol) and dialdehydes during gel formation probed by H n.m.r.--a kinetic study. Polymer 1997, 38 (19), 4863-4871. DOI: 10.1016/S0032-3861(97)00002-5.[7] Roberts, I.; Kimball, G. E., The Halogenation of Ethylenes. Journal of the American Chemical Society 1937, 59 (5), 947-948. DOI: 10.1021/ja01284a507[8] George, A. O.; Arpad, M., Halogen Addition. In Hydrocarbon Chemistry, 2 ed.; John Wiley & Sons, Inc.: Canada, 2003; Vol. 1, pp 290-312[9] Moulay, S., Molecular iodine/polymer complexes. In Journal of Polymer Engineering, 2013; Vol. 33, p 389. DOI: 10.1515/polyeng-2012-0122[10] Majibur Rahman Khan, M.; Gotoh, Y.; Morikawa, H.; Miura, M., Surface Morphology and Properties of Bombyx mori Silk Fibroin Fiber Treated with I 2-KI Aqueous Solution. Textile Research Journal 2009, 79 (14), 1305-1311 Keywords: polymer, clinical application, biomedical application, Biodegradable material Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Imaging with biomaterials Citation: Francis NK, Pawar H, Ghosh P and Dhara S (2016). 2, 5-Dimethoxy-2, 5-Dihydro-Furan (DMDF) crosslinked radio-opaque biodegradable antimicrobial sutures. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00787 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. 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