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First Report of ‘ Candidatus Phytoplasma solani’ Associated With Potato Stolbur Disease in Montenegro

2016; American Phytopathological Society; Volume: 100; Issue: 8 Linguagem: Inglês

10.1094/pdis-02-16-0180-pdn

1943-7692

Sanja Radonjić, Snježana Hrnčić, Andrea Kosovac, Oliver Krstić, Milana Mitrović, Jelena Jović, Ivo Toševski,

Plant Pathogenic Bacteria Studies

HomePlant DiseaseVol. 100, No. 8First Report of ‘Candidatus Phytoplasma solani’ Associated With Potato Stolbur Disease in Montenegro PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of ‘Candidatus Phytoplasma solani’ Associated With Potato Stolbur Disease in MontenegroS. Radonjić, S. Hrnčić, A. Kosovac, O. Krstić, M. Mitrović, J. Jović, and I. ToševskiS. RadonjićSearch for more papers by this author, S. HrnčićSearch for more papers by this author, A. KosovacSearch for more papers by this author, O. KrstićSearch for more papers by this author, M. MitrovićSearch for more papers by this author, J. JovićSearch for more papers by this author, and I. ToševskiSearch for more papers by this authorAffiliationsAuthors and Affiliations S. Radonjić S. Hrnčić , University of Montenegro, Biotechnical Faculty, Centre for Plant Protection, 81000 Podgorica, Montenegro A. Kosovac O. Krstić M. Mitrović J. Jović , Institute for Plant Protection and Environment, Department of Plant Pests, 11080 Zemun, Serbia I. Toševski , Institute for Plant Protection and Environment, Department of Plant Pests, 11080 Zemun, Serbia, and CABI, 2800 Delémont, Switzerland. Published Online:24 May 2016https://doi.org/10.1094/PDIS-02-16-0180-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Current data on the occurrence of plant diseases associated with ‘Candidatus Phytoplasma solani’ (16SrXII-A subgroup) in Montenegro apply only to grapevine (Kosovac et al. 2016; Radonjić et al. 2009). Potato (Solanum tuberosum), as an important food crop and export commodity of Montenegro, is being monitored regularly for plant diseases that can disrupt field production. Potato stolbur disease caused by ‘Ca. P. solani’ has already been reported throughout Europe and in neighboring Serbia (Jović et al. 2011; Mitrović et al. 2016). Field and molecular data were used to verify association of this pathogen with symptomatic potato plants in Montenegro. During July and August 2015, leaves with petioles from 14 symptomatic potato plants were collected at two locations in central Montenegro: Nikšić (42°50.29 N, 18°55.57′ E) and Danilovgrad (42°33.41′ N, 19°05.46′ E). All plants exhibited symptoms previously reported as typically associated with potato stolbur disease, such as leaf redness and yellowing, swollen stems, and aerial tuber formation (Mitrović et al. 2016). Approximately 10 to 15% of plants on both locations were symptomatic at the time of collection. In addition, 10 asymptomatic plants were sampled and analyzed as controls. Identification of ‘Ca. P. solani’ was conducted following the stol11 stolbur-specific protocol (Radonjić et al. 2009), while characterization of isolates was performed applying multilocus sequence typing of tuf, secY, stamp, and vmp1 marker genes (Cvrković et al. 2014). Sequences of the amplified genes were deposited in GenBank under accession numbers KU588188 to 93. The presence of ‘Ca. P. solani’ was detected in 10 of 14 symptomatic potatoes, all with symptoms of yellowing and areal tuber formation, while all control plants tested negative. The RFLP analysis of tuf gene nested PCR products using HpaII endonuclease (Fermentas) revealed uniform tuf-b type in all positive samples. Nucleotide sequence analyzes of secY and stamp amplicons identified single genotype on each of the genes, identical to the reference strain STOL for secY (AM992086) and Rqg50 for stamp (KC703019). However, amplification of the vmp1 gene was obtained in only seven samples and RsaI restriction generated two profiles: V14 (3 isolates) and V17 (4 isolates). Subsequent sequencing and comparison with reference vmp1 strains revealed closest relatedness with Mp46 isolate of the V14 profile (HM008606), and CrHo12_721 isolate of the V17 profile, i.e., Vm_At7 genotype (KJ469731). Multilocus tuf/stamp/vmp1 genotype of tuf-b/Rqg50/V17 was previously detected in Montenegro in association with Convolvulus arvensis, Vitex agnus-castus, and their corresponding Hyalesthes obsoletus population of vector (Kosovac et al. 2016), while tuf-b/Rqg50/V14 genotype is reported to infect symptomatic potato plants in Serbia (Mitrović et al. 2016). Presence of potato stolbur disease in Montenegro is an important finding with potential far-reaching consequences, requiring further research to determine the inoculum source plants and insect vector populations with the aim of devising and implementing effective vector control strategies.References:Cvrković, T., et al. 2014. Plant Pathol. 63:42. https://doi.org/10.1111/ppa.12080 Crossref, ISI, Google ScholarJović, J., et al. 2011. B. Insectol. 64:S83. ISI, Google ScholarKosovac, A., et al. 2016. Plant Pathol. 65:285. https://doi.org/10.1111/ppa.12409 Crossref, ISI, Google ScholarMitrović, M., et al. 2016. Eur. J. Plant Pathol. 144:619. https://doi.org/10.1007/s10658-015-0800-y Crossref, ISI, Google ScholarRadonjić, S., et al. 2009. J. Phytopathol. 157:682. https://doi.org/10.1111/j.1439-0434.2009.01560.x Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 8 August 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 22 Jul 2016Published: 24 May 2016First Look: 28 Mar 2016Accepted: 23 Mar 2016 Pages: 1775-1775 Information© 2016 The American Phytopathological SocietyCited byCandidatus Phytoplasma solani (Stolbur phytoplasma)CABI Compendium, Vol. CABI CompendiumMultilocus Genetic Characterization of Phytoplasmas11 November 2019Phytoplasmas Infecting Vegetable, Pulse and Oil Crops5 September 2018