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BIBTEX RIS

Development and characterization of DNA microsatellite primers for buriti (Mauritia flexuosa L.f.)

2012; Research Foundation of Ribeirão Preto; Volume: 11; Issue: 4 Linguagem: Inglês

10.4238/2012.september.17.6

ISSN

1676-5680

Autores

Elytânia Veiga Menezes, W.F.S. Souto, A. Y. Ciampi, Vânia Azevedo, Henrique Maia Valério, Márcio Antônio Silva Pimenta,

Tópico(s)

Silkworms and Sericulture Research

Resumo

Mauritia flexuosa L. (Arecaceae) is a palm tree species known as buriti that occurs in the Cerrado biome.It is characteristic of the vereda, a typical ecosystem of central Brazil.In this phytophysiognomy, M. flexuosa and other groups of arboreal-herbaceous species develop in open fields with very humid soils.M. flexuosa can be found in forest borders and is a palm tree with a wide distribution in South America (Brazil, Colombia, Venezuela, French Guyana Ecuador, Peru, and Bolivia).The main objectives of this study were to develop simple sequence repeat marker-enriched libraries and to characterize these loci in buriti palm to facilitate future population studies.A total of 40 sequences derived from the microsatellite-enriched libraries were selected for primer design.The optimization results showed that 9 primer pairs could successfully amplify polymorphic target fragments of the expected sizes.The data also show that the described primers ©FUNPEC-RP www.funpecrp.com.

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