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Reversible activation of c-Myc in thymocytes enhances positive selection and induces proliferation and apoptosis in vitro

2000; Springer Nature; Volume: 19; Issue: 15 Linguagem: Inglês

10.1038/sj.onc.1203508

1476-5594

Bettina Rudolph, Anne‐Odile Hueber, Gérard I. Evan,

Invertebrate Immune Response Mechanisms

In order to study the effect of c-Myc activation in T lymphocytes in vivo, we generated transgenic mice that express a 4-hydroxytamoxifen (4-OHT)-dependent switchable c-myc oncoprotein under the control of the proximal lck promoter. Activation of c-MycERTM causes no obvious alteration in T cell ontogeny. However, using MHC class I restricted H-Y-TCR transgenic mice, we found that c-Myc activation in vivo enhances the efficiency of positive selection. Moreover, splenic T cells derived from lck-c-mycERTM transgenic mice in which c-Myc had been activated exhibited increased proliferation in vitro in response to activation with anti-CD3/CD28 antibody. Activation of c-MycERTM also promotes apoptosis in thymocytes in vitro.