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Molecular variability of a collection of Dioscorea polygonoides (Willd.) Humb. and Bonpl. (wild yam) of the Gran Caldas region (Colombia) using AFLPs

2006; Taylor & Francis; Volume: 81; Issue: 5 Linguagem: Inglês

10.1080/14620316.2006.11512141

2380-4084

Jaime Niño, María José Díez, Fernando Nuez,

Phytochemical Studies and Bioactivities

SummaryAmplified fragment length polymorphism (AFLP) analysis was used to assess intra-specific variability and relationships in wild yam (Dioscorea polygonoides). A total of 67 accessions from different locations in The Gran Caldas Region of Colombia, were analysed, and 464 polymorphic AFLP fragments were obtained using three primer combinations. Pairwise genetic similarities were estimated using the Dice similarity coefficient. The resulting genetic similarity matrices were used to generate unweighted pair group method using arithmetic mean (UPGMA) dendrograms.A principal coordinates analysis (PCoA), based on genetic similarity matrices, was also performed based on the 464 polymorphic AFLP fragments. Both methods used to evaluate the data gave congruent results, leading to similar groupings that reflected the geographical origins of the accessions. According to the PCoA, Ucumarí Park accessions formed a distinct isolated group (Group I), while the Circasia and Salamina accessions constituted a distant group (Group II). In addition, Group IV contained those D. alata accessions that were used as an outgroup, and was equidistant from Groups I and II by PCoA analysis. Finally, accessions of uncertain origin obtained from Botanic Gardens grouped in Group III. Our results confirm that AFLP analysis is a reliable method with which to search for the degree of intra-specific variability and relationships among accessions of D. polygonoides.