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Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Childhood B Lineage Acute Lymphoblastic Leukemia Clonality Study by the Polymerase Chain Reaction

1997; Lippincott Williams & Wilkins; Volume: 19; Issue: 6 Linguagem: Inglês

10.1097/00043426-199711000-00005

1536-3678

Carlos Alberto Scrideli, Aguinaldo Luíz Simões, Ricardo Defavery, José Eduardo Bernardes, Maria Herbenia Oliveira Duarte, Luíz Gonzaga Tone,

T-cell and Retrovirus Studies

Purpose B cell precursors acute lymphoblastic leukemia (ALL) present rearrangements in the heavy chain immunoglobulin and T cell receptor genes, especially in the complementarity determining region 3 (CDR-3) and T cell receptor δ (TCRδ) (Vδ2Dδ3) regions. These rearrangements may be amplified by the polymerase chain reaction (PCR) and used as clonal markers of B lineage ALL. Our purpose was to study clonality at the DNA level by PCR in B lineage ALL. Patients and Methods Fifty-three pediatric patients (36 with B lineage ALL, 7 with ALL-T, and 10 with nonlymphocytic disease) were investigated using consensus primers for the CDR-3 regions of IgH and TCRδ. Results Clonality was detected in 86.1% of the patients with B lineage ALL when the primers for the CDR-3 regions were used, in 41.6% when the primers for TCRδ were used, and in 91.6% when the two primers were used together. Biclonality was found in 22.5% and 6.6% of patients that have shown clonality for CDR-3 and TCRδ, respectively. Clonality was not detected in any other samples using these primers. Conclusions PCR using CDR-3 and TCRδ primers can be used as an aid for B lineage ALL diagnosis and clonal evolution of theses disease.