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Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration

2014; American Chemical Society; Volume: 15; Issue: 6 Linguagem: Inglês

10.1021/bm500168s

1526-4602

Agnieszka K. Muszanska, Edward T.J. Rochford, Agnieszka Gruszka, Andreas A. Bastian, Henk J. Busscher, Willem Norde, Henny C. van der Mei, Andreas Herrmann,

Antimicrobial agents and applications

This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.