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Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status

2012; Inter-Research; Volume: 457; Linguagem: Inglês

10.3354/meps09711

1616-1599

Audrey Caro, Arthur Escalas, Christophe Bouvier, Evelyse Grousset, Nicole Lautrédou-Audouy, Cécile Roques, M. Charmantier, Olivier Gros,

Protist diversity and phylogeny

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 457:11-27 (2012) - DOI: https://doi.org/10.3354/meps09711 Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status Audrey Caro1,*, Arthur Escalas1, Corinne Bouvier1, Evelyse Grousset1, Nicole Lautredou-Audouy2, Cécile Roques1, Mireille Charmantier1, Olivier Gros3 1UMR-CNRS 5119, Laboratoire Ecologie des Systèmes Marins Côtiers, Université Montpellier II, 34095 Montpellier, Cedex 5, France 2INSERM U554, CNRS UMR 5048, Montpellier, France 3UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe 'Biologie de la mangrove', BP 592, Université des Antilles et de la Guyane, 97159 Pointe-à-Pitre Cedex, Guadeloupe, France *Email: audrey.caro@univ-montp2.fr ABSTRACT: Sphaeroma serratum is a marine isopod species that inhabits seashores from Europe to West Africa. The individuals live under stones in direct contact with reduced sediments and harbour a diverse bacterial community on the cuticle of their pleopods. We investigated the diversity of these epibiotic bacteria on male (pubescent and senescent) and female specimens with electron microscopic observations and molecular tools. The microbial community of S. serratum was shown to be composed of at least 5 bacterial morphotypes observed on the pleopodal cuticle in all male specimens. Using fluorescence in situ hybridization, we identified 5 major phylogenetic groups (α-, β-, γ- and δ-Proteobacteria and Archaea) whereas denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments of epibiotic bacteria revealed 50 bands. The bacterial community associated with S. serratum seems more diverse than in other marine crustaceans, such as Rimicaris. The relative diversity of this bacterial community was also studied in relation to the molt cycle. The comparison of DGGE band patterns of several individuals from female, pubescent male and senescent male groups revealed that the bacterial community diversity was dependent on the sex and the age of the individuals and more generally on the molt status. KEY WORDS: Sphaeroma · Molt cycle · Epibiotic biofilm · DGGE band pattern · FISH · Crustacean Full text in pdf format PreviousNextCite this article as: Caro A, Escalas A, Bouvier C, Grousset E and others (2012) Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status. Mar Ecol Prog Ser 457:11-27. https://doi.org/10.3354/meps09711 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 457. Online publication date: June 21, 2012 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2012 Inter-Research.