Modification of Radiation Response of CHO Cells by Methyl-Isobutyl Xanthine: 1. Reduction of D 0
1979; Radiation Research Society; Volume: 78; Issue: 1 Linguagem: Inglês
10.2307/3575002
ISSN1938-5404
Autores Tópico(s)Cancer therapeutics and mechanisms
ResumoMethyl-isobutyl xanthine (MIX), an inhibitor of cyclic AMP phosphodiesterase, induces transient elevation of intracellular cyclic AMP in CHO cells when added to the medium. Reduction of Do of irradiated, exponentially growing CHO cells is observed when MIX is added to the medium shortly before or shortly after irradiation, or when dibutyryl cyclic AMP or prostaglandin El are added after irradiation. Results of the latter experiment and of experiments in which the time of MIX addition was varied in relation to the time of irradiation suggested that an important factor in reducing Do in MIX-treated irradiated cells was the presence of an elevated concentration of intracellular cyclic AMP after irradiation. MIX-treated cells showed reduction in survival when irradiated with high doses in late G1 and S phase, but no reduction
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