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SEQUENCE ANALYSIS OF V3 LOOP REGION OF HIV-1 STRAINS PREVALENT IN INDIA

1993; National Institute of Infectious Diseases; Volume: 46; Issue: 2 Linguagem: Inglês

10.7883/yoken1952.46.95

1884-2828

H Tsuchie, Takashi Kurimura, Namiko Yoshihara, Takashi Kitamura, Mitsunobu Imai, J K Maniar,

Monoclonal and Polyclonal Antibodies Research

The third variable (V3) domain of human immunodeficiency virus type 1 (HIV-1) env gene has been found to elicit type-specific neutralizing antibodies as well as a cytotoxic and helper T-cell response in both humans and animals. We analyzed the V3 domain of 8 HIV-1 isolates from India by using polymerase chain reactions. The V3 loops of 7 Indian isolates contained the apical tetra peptide GPGQ, while the V3 loop of one Indian isolate carried the apical tetra peptide-GPGK. The amino acid sequences of the seven Indian isolates were closely related to each other, with an average of the nucleotide sequence homology of 96.0% (94.6 to 97.6%) . The marked relatedness of the amino acid sequences among the seven Indian HIV-1 isolates indicated a recent and very rapid spread of this HIV-1 variant in Bombay. The amino acid sequence of the C2/V3 region of env gene of the 7 Indian isolates were homologous to the C subtype reported by Meyers et al. These findings could be useful in assessing the sources of infection and developing an AIDS vaccine.