Effects of water-holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture
2013; Wiley; Volume: 102; Issue: 1 Linguagem: Inglês
10.1002/jbm.a.34695
ISSN1552-4965
AutoresWakana Togami, Akira Sei, Tatsuya Okada, Takuya Taniwaki, Toru Fujimoto, Takayuki Nakamura, Shogo Tahata, Yoshitaka NAKANISHI, Hiroshi Mizuta,
Tópico(s)Silk-based biomaterials and applications
ResumoJournal of Biomedical Materials Research Part AVolume 102, Issue 1 p. 247-253 Original Article Effects of water-holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture Wakana Togami, Wakana Togami Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorAkira Sei, Akira Sei Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorTatsuya Okada, Tatsuya Okada Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorTakuya Taniwaki, Takuya Taniwaki Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorToru Fujimoto, Toru Fujimoto Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorTakayuki Nakamura, Takayuki Nakamura Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorShogo Tahata, Shogo Tahata Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorYoshitaka Nakanishi, Yoshitaka Nakanishi Department of Advanced Mechanical Systems, Graduate School of Science and Technology, Kumamoto University, 1 Honjo Chuo-ku, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorHiroshi Mizuta, Hiroshi Mizuta Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this author Wakana Togami, Wakana Togami Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorAkira Sei, Akira Sei Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorTatsuya Okada, Tatsuya Okada Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorTakuya Taniwaki, Takuya Taniwaki Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorToru Fujimoto, Toru Fujimoto Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorTakayuki Nakamura, Takayuki Nakamura Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorShogo Tahata, Shogo Tahata Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorYoshitaka Nakanishi, Yoshitaka Nakanishi Department of Advanced Mechanical Systems, Graduate School of Science and Technology, Kumamoto University, 1 Honjo Chuo-ku, Kumamoto-city, 860–8556 JapanSearch for more papers by this authorHiroshi Mizuta, Hiroshi Mizuta Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860–8556 JapanSearch for more papers by this author First published: 09 May 2013 https://doi.org/10.1002/jbm.a.34695Citations: 6 Correspondence to: A. Sei; e-mail: a1203ki@fc.kuh.kumamoto-u.ac.jp Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The aim of the study is to estimate the effects of the water-holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran-coated PVF sponges with or without water-holding capability. The BMCs seeded onto the PVF sponges with water-holding capability showed more significant increases in DNA content, alkaline phosphatase (ALP) activity, osteocalcin content, and calcium deposition than those without water-holding capability. These results suggest that the Dextran-coated PVF sponges with high water-holding capability would have potential uses as both a new scaffold to bone tissue engineering and as a new biomaterial. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 247–253, 2014. Citing Literature Volume102, Issue1January 2014Pages 247-253 RelatedInformation
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