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Selective Detection and Quantification of Modified DNA with Solid-State Nanopores

2014; American Chemical Society; Volume: 14; Issue: 10 Linguagem: Inglês

10.1021/nl501340d

1530-6992

Autumn Carlsen, Osama K. Zahid, Jan Růžička, Ethan Will Taylor, Adam R. Hall,

Microfluidic and Bio-sensing Technologies

We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein–DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.